Case Study 1

Nutrient Content Comparison, Reaching Conventional Levels

Using chicken manure and vermicompost extracts, organic liquid nutrient solution was brewed, achieving nutrient content similar as of conventional hydroponic nutrient solution based on the Hoagland recipe, achieving all recommended range of nutrients for hydroponic cultivation.

Blue and brown Bar Graph showing nutrients levels of plants
Green Bar Graph showing nutrients levels of plants
Case Study 2

Replacing Nitrogen Demand With Residual Organic Matter

A hydroponic grower having a nearby chicken farm. Using our service, a weekly fertilizer can be brewed from locally available organic nutrients. Replacing the Nitrogen supply of a conventional hydroponic recipe with precisely processed manure extract, 15% mass increase was achieved for Basil.

Bar Graph about the Average Plant Fresh Mass in three different trials

T1 : Only chicken manure and vermicompost extracts
T2: Nitrogen sourced from chicken manure in a modified Hoagland recipe
T3: Hoagland nutrient solution







Case Study 3

Generic Synthetic Fertilizers
vs
Circulus Agtech’s Fertilizer - their effects on Corn

Circulus Agtech ran running a pilot field trial in Lunenburg, Ontario to evaluate our frass-derived liquid fertilizer as a sustainable alternative to synthetic nitrogen. The study is structured around four treatments: conventional urea, and plots where 50–75% of urea nitrogen is substituted with our liquid fertilizer. Phosphorus and potassium are balanced across all plots using standard amendments to ensure fair comparisons.Fertilizer is applied in multiple rounds over the season, with growth, yield, and nitrogen use efficiency carefully tracked. This trial will help us quantify how well our solution supports staple crops like corn while reducing dependence on synthetic fertilizers. Early results will guide both agronomic recommendations and future scale-up for Canadian growers.

Intermediary Results clearly showing the 50:50 and 75:25 ratios of Frass Fertilizer to Conventional synthetics

Final results showing stronger results the more Frass fertilizer is used. There was a negative slope in the direction of control 2, as the Frass applications were in liquid form, leaching likely occurred in this direction. Therefore, without leaching, the 75:25 plot would have likely out performed the other






Case Study 4

Generic Synthetic Fertilizers
vs
Frass and Cow manure based Fertilizers -
their effect on Marigolds

Circulus Agtech conducted a greenhouse trial to evaluate our frass-derived liquid fertilizer on marigolds grown in hydroponic systems. Plants were transplanted in June 2025 and monitored for pH and EC adjustments throughout the season using municipal water balanced with acidulants.


These diagrams describe the process, 4 trays with different fertilizer sources but same nutrient concentrations, water volumes and pH levels each containing and fertilizing 13 Marigolds per tray. The independent variable being assessed is the impacts of differing sources of Circulus Agtech's fertilizer on the results for nutrient senestve flowers. Two Frass based fertilizer batches were generated by the extraction the nitrates from 1 batch of Frass twice, their results were compared to those of Cow Manure and a Control which served as a reference for the overall success of Circulus Agtech's products on commercial flowers compares to conventional fertilizations.

Though Conventional Fertilizers produced more flowers than circulus Agtech's product, the Frass 1st extract batch produced the most high quality ones.







Case Study 5

Frass based Biostimulant - its effect on Pepper Plants

Circulus Agtech ran biotimulant trial test on paper plants. The pepper plants were given the same quantity of Marco and Micro nutrients, water and pH environments, the only independent variable was that half the plants were exposed to the Frass Based Biotimulant. The nutrients added by the biostimulant were compensented for in the batch without them.

Pepper plants Without Biostimulant on the left vs pepper plants With Biostimulant on the right (Visually much bigger and later generates many more peppers)


Case Study 6

Pepper plant growth and fruit production under different fertilization treatments

This trial evaluates pepper plant growth and fruit production under different fertilization treatments, including multiple frass-based (insect manure) formulations and a conventional synthetic control.

20% more sugar in peppers produced by Circulus’ frass-based fertilizer. Comparable yield and plant growthbetween synthetic fertilizer and frass-based fertilizer.







Case Study 7

Comparative analysis studying the impact of three organic digestates supplemented with inorganic fertilizers:  Frass Digestate (FD), Cow Manure Digestate (CMD), and Cucumber Leaf Digestate (CLD)

All treatments yielded similar plant heights and biomass. CLD treatments demonstrated superior nutrient use efficiencies as well as higher chlorophyll b content and the highest shoot and root dry mass.

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